heterospermic insemination performed by frozen-thawed and liquid stored boar sperm

Frydrychová S., Lustyková A., Lipenský J., Seifert J., Kuchařová S., Truněčková J., Rozkot M.

Abstract

   The objective of this study was to verify the fertility of cryopreserved boar sperm by heterospermic insemination performed by a mix of a liquid stored and post-thaw sperm. The second objective of this study was to utilize the benefits of a liquid stored insemination doses to reduce a negative impact on fertility results after post-thaw insemination performed by cryopreserved sperm. Eight sperm rich fraction from seven fertile hybrid boars with verified fertilizing ability were collected by the gloved-hand technique. Semen from five boars was cryopreserved and semen from two boars was liquid stored. Post-thaw sperm with 20-30% progressive motility and liquid stored sperm evaluated as a sperm with substandard quality were used to produce heterospermic insemination doses. In total, 14 sows were inseminated using the intrauterine technique. Piglet paternity was determined by molecular-genetic analysis of microsatellite markers. The results of paternity showed that the sires of all the born piglets were boars used as producers of liquid stored sperm. As expected, the negative impact of insemination performed by cryopreserved sperm on sow fertility results was reduced by heterospermic insemination performed by a mix of a liquid stored and post-thaw sperm. However, no piglets from cryopreserved sperm were born. Cryopreserved sperm was unable to compete with liquid stored sperm in heterospermic insemination doses.

Key Words: Boar semen, heterospermy, artificial insemination, cryopreservation