Effect OF THE NEW LIQUID CONCENTRATE EXTENDER ON THE BOAR SEMEN QUALITY COMPARED TO THE TRADITIONAL BTS EXTENDER
Frydrychová S., Lustyková A., Lipenský J., Seifert J., Kuchařová S., Truněčková J., Rozkot M.
The objective of this study was to evaluate the boar semen quality in newly development liquid extender concentrate K2 compared to traditional BTS extender. Fifteen sperm rich fractions from 5 boars from one AI centre with motility ≥80 % and the number of morphologically abnormal spermatozoa ≤25 % were used in this study. Semen were diluted 1+4 in extenders K2 and BTS and stored at 17°C up to 72h storage time. The boar semen quality was evaluated by sperm motility, sperm viability, short hypoosmotic swelling test (sHOST) and long-term thermoresistance survival test (TRT). In total progressive motility were recorded differences between BTS (76.75 %) and K2 (67.65 %) extender (P<0.05). In sperm viability and sHOST were not found differences between extenders (P>0.05). In TRT test were noted differences (P<0.05) in sperm motility between BTS and K2 at 24h (55.24 % and 75.71 %) and 48h storage time (52.86 % and 63.33 %), in 1h (64.52 % and 75.48 %) and 5h (38.33 % and 50.24 %) of monitoring and in total sperm motility (52 % and 61 %). Boar semen quality were effected by extenders, storage time and hours of monitoring (P<0.05). In conclusion, according results is suitable used K2 extender into 48h storage time because than sperm motility is decreased. Further was found that BTS extender had worse thermo-resistance stability in TRT test during monitoring hour compared to K2.
Key Words: Boar semen, extender, storage time, sperm quality