Aim of this study was to determine the concentration of gross energy in sow’s colostrum. Samples of colostrum were taken from 8 sows (White improved), which were at different farrow (1st to 4th). Sows were fed with feed mixture for lactating sows. Colostrum samples were taken per hand without injection of oxytocin from different mammary glands and in different time periods (0th; 1st; 2nd; 3th; 4th; 5th; 6th; 12th and 18th hour). Than were the colostrum samples lyophilized. The concentration of gross energy (GE) in colostrum samples was performed by Automatic Calorimeter AC500. Values of GE in samples of sows colostrum were analyzed by one-way ANOVA, the differences in average GE concentration of colostrum between different sampling times were tested with T-test (P<0.05) using a SAS system. We found statistically significance (P<0.05) in dry matter concentration and GE concentration in kg of dry matter between first sample (0.hour - took at time of birth of first piglet) and samples took at 12th hour from birth of first piglet. Concentration of GE in colostrum during parturition to 6th hour of lactation was in range from 5.14 to 5.51 MJ.kg-1. After 6th hour to 18th hour (4.46 MJ.kg-1) we detected decrease in gross energy concentration in colostrum.